Platform overview and consultation
Potential new users are provided an overview of how the platform works and methodologies used in the lab.
All prospective users begin with a consultation to discuss their project plan and identify feasibility for use of the platform. Contact Shanu Mehta to schedule a consultation.
Nanowell devices
Users may purchase nanowell devices for use in their laboratories.
- PDMS Standard configuration (1”x3” slide; 50 * 50 * 100 micron wells) for microengraving or imaging
- SeqWell array (1”x3” slide; 50 * 50 * 65 micron wells) + membrane: includes a functionalized array and membrane; no beads
- Functionalized SeqWell array: includes a functionalized array only; no membrane or beads (requires access to a plasma cleaner on the user's end)
Live Cell Imaging and Analysis - Incucyte S3
The Incucyte S3 system allows automatic image acquisition and analysis of cells in a physiologically relevant environment.
Key features:
- Six microplates in parallel
- Three interchangeable vessel trays compatible with flasks, dishes and microplates
- Two color Green/Red plus HD phase contrast imaging
- 4X, 10X, 20X objective
Prior to first use of this instrument, please contact Shanu Mehta to schedule orientation and training. The full training will cover experimental set up and preliminary data analysis.
Once trained, investigators will be granted permissions to reserve the instrument for independent use.
SeqWell sample processing
The Core offers training and assisted use of the SeqWell platform. All users must set up a complimentary initial consultation to discuss project scope and feasibility for platform use. Contact Shanu Mehta to set up a consultation.
Single-cell retrieval
We use a robotic micromanipulator (CellCelector) for robust, high throughput single cell picking. The system offers bright field and fluorescence channels (DAPI, APC, PE), to aid in isolation based on expression of fluorescence markers or cell morphology. We offer in-depth user training for independent use of the system as well as fully staff-assisted use of equipment. (More details on equipment can be found here).
Laser Scanner for Slide Microarrays
The Genepix 4400 system from Molecular Devices is available to users for assisted use, training as well as independent use.
Available lasers: Red, Blue, Green & Yellow (635, 488, 635a and 594nm).