Users are encouraged to consult with Core staff in all microscopy methods.

Users are expected to be familiar with basic immunostaining and fixation techniques and slide preparation. A common method to prepare samples is available here.

The Fluorescent Protein database has several useful tools for choosing the right fluorophore for your experiment. It includes a spectra viewer, FRET efficiency calculators, and microscope fluorophore efficiency reports. We will be adding KI-MIT microscope configurations to the database over time.

Additional methodology resources


General protocols for microscopy



Image acquisition and processing–deconvolution

Fluorescent probes and antibodies

Fluorescence spectra viewer

Optical filters

Imaging chambers