Methods

Users are encouraged to consult with Core staff in all microscopy methods.

Users are expected to be familiar with basic immunostaining and fixation techniques and slide preparation. A common method to prepare samples is available here.

The Fluorescent Protein database has several useful tools for choosing the right fluorophore for your experiment. It includes a spectra viewer, FRET efficiency calculators, and microscope fluorophore efficiency reports. We will be adding KI-MIT microscope configurations to the database over time.

Additional methodology resources
 

Organizations

General protocols for microscopy

Microscopes

Lasers

Image acquisition and processing–deconvolution

Fluorescent probes and antibodies

Fluorescence spectra viewer

Optical filters

Imaging chambers