DeltaVision Ultra Microscope

DeltaVision Ultra Microscope

Overview

  • Deconvolution microscope for long-term 3D live-cell acquisition
  • Multiwell acquisition

Specifications

  • Inverted microscope with XYZ nano-motion stage and 3D deconvolution
  • Well-plate scanning
  • Environmental chamber with temperature control and CO2 enrichment
  • Solid state LED illumination module
  • LED transmitted light source
  • SoftWorx software for image acquisition and deconvolution
  • Sensitive back-thinned sCMOS camera
  • Ultimate Focus module corrects focus drift over time

Fluorescence Filters

Name Set Std dyes Cy series Alexa Ex Em
Blue B-G-O-FR DAPI/Hoechst/BV421     390 435
Green B-G-O-FR FITC/GFP Cy2 AF488 475 525
Orange B-G-O-FR TRITC Cy3 AF546 542 597
Far-Red B-G-O-FR APC Cy5/Cy5.5 AF647 632 679
             
Blue B-G-R-FR DAPI/Hoechst/BV421     390 435
Green B-G-R-FR FITC/GFP Cy2 AF488 475 525
Red B-G-R-FR RFP/TxRed Cy3.5 AF594 575 625
Far-Red B-G-R-FR APC Cy5/Cy5.5 AF647 632 679
             
Cyan C-Y-R CFP     438 475
Yellow C-Y-R YFP/TRITC   AF514 513 548
Red C-Y-R mCherry/TxRed Cy3.5 AF594 575 625

Objectives

Objective OIL Corr WD mm Z DOF µm
4x/0.10   Plan N 18.50 71.25
10x/0.40   UPlan Superapochromat 3.10 5.06
20x/0.75   UPlan Superapochromat 0.60 1.41
40x/1.30 Oil UPlan Fluor N 0.20 0.68
60x/1.40 Oil UPlan Apochromat 0.11 0.54
100x/1.40 Oil UPlan Superapochromat 0.13 0.50