DeltaVision Ultimate Focus Microscope with TIRF Module

Overview

  • Deconvolution microscope for 3D live-cell acquisition.
  • TIRF (Total Internal Reflection Fluorescence) module for single-molecule imaging near the coverslip surface.
  • Photokinetics laser mode allows for FRAP (Fluorescence Recovery After Photobleaching Experiments)

Specifications

  • Inverted Olympus X71 microscope with XYZ nano-motion stage with 18 mm of XY travel.
    NOTE: Stage travel is limited to an 18 x 18 mm area
  • Environmental chamber with temperature control and CO2 enrichment
  • Solid state LED illumination module
  • Four lasers for TIRF and Photokinetics
  • SoftWorx software for image acquisition and deconvolution
  • Sensitive back-thinned sCMOS camera
  • Ultimate Focus module corrects focus drift over time

Fluorescence Filters and Lasers

Name Set Std dyes Cy series Alexa Ex Em
DAPI Std DAPI/Hoechst/BV421     390 435
FITC Std FITC/GFP Cy2 AF488 475 523
TRITC Std TRITC Cy3 AF546 542 594
CY-5 Std APC Cy5/Cy5.5 AF647 632 676
             
DAPI Alexa DAPI/Hoechst/BV421     390 435
FITC Alexa FITC/GFP Cy2 AF488 475 523
A594 Alexa RFP/TxRed Cy3.5 AF594 575 632
CY-5 Alexa APC Cy5/Cy5.5 AF647 632 676
             
CFP Live CFP     438 470
GFP Live GFP Cy2 AF488 475 525
YFP Live YFP/TRITC   AF514 513 559
mCherry Live mCherry/TxRed Cy3.5 AF594 575 632
             
405 Laser TIRF/PK DAPI/Hoechst/BV421     405 435
488 Laser TIRF/PK FITC Cy2 AF488 488 523
514 Laser TIRF/PK YFP/TRITC   AF514 514 632
561 Laser TIRF/PK TRITC/TxRed Cy3/Cy3.5 AF546/594 561 676

Objectives

Objective OIL Corr WD mm Z DOF µm
10x/0.40   UPlan Apochromat 3.10 5.06
20x/0.75   UApochromat / 340 0.55 1.41
40x/0.85   UPlan Apochromat 0.20 0.95
60x/1.40 Oil Plan Apochromat 0.15 0.54
100x/1.49 TIRF Oil Apochromat N TIRF 0.09 - 0.16 0.44