Resource overview & consultation
All prospective users begin with an overview of available imaging capabilities and a consultation to discuss project goals and feasibility. Contact Shanu Mehta to schedule a consultation.
All new users should submit this form 24h before their appointment.
Live cell imaging & analysis: Incucyte S3
The Incucyte S3 system allows automatic image acquisition and analysis of cells in a physiologically relevant environment.
Key features:
- Six microplates in parallel
- Three interchangeable vessel trays compatible with flasks, dishes and microplates
- Two color Green/Red plus HD phase contrast imaging
- 4X, 10X, 20X objective
Prior to first use of this instrument, please contact Shanu Mehta to schedule orientation and training. The full training will cover experimental set up and preliminary data analysis.
Once trained, investigators will be granted permissions to reserve the instrument for independent use.
Live cell imaging and analysis: Nanolive 3D Cell Explorer 96focus
Nanolive's microscope allows automated, label-free live cell imaging and analysis in ambient conditions.
Key features:
- Upto four 35mm dishes or one 96-well microplate sample capacity
- Optional: four fluorescence channels
- Fully integrated digital analysis
- High-content, multiplexed data
Single-cell imaging and retrieval
We use a robotic micromanipulator (CellCelector) for robust, high throughput single cell picking. The system offers bright field and fluorescence channels (DAPI, APC, PE), to aid in isolation based on expression of fluorescence markers or cell morphology. We offer in-depth user training for independent use of the system as well as fully staff-assisted use of equipment. (More details on equipment can be found on the manufacturer's website.
Laser scanner for slide microarrays
The Genepix 4400 system from Molecular Devices is available to users for assisted use, training as well as independent use.
Available lasers: Red, Blue, Green & Yellow (635, 488, 635a and 594nm).