Olympus FV1200 Laser Scanning Confocal Microscope
Overview
Specifications
- Inverted Olympus IX83 microscope
- Olympus XYZ Motorized stage
- Stage incubator with heat
- 3 PMTs and 2 High Sensitivity Detectors
- Optional transmitted light detector
- Image sizes up to 1024 x 1024 pixels
- Scanning speed: (pixel time): 2 µs - 5 ms/High speed scanning mode: 16 frames/s (256x256)
- Silicone Immersion Objectives for deeper tissue imaging
- Olympus acquisition software
Fluorescence Filters and Lasers
| Name |
Set |
Std dyes |
Cy series |
Alexa |
Ex |
Em |
| 405 Laser |
Confocal |
DAPI/Hoechst |
|
|
405 |
461 |
| 440 Laser |
Confocal |
CFP |
|
|
440 |
480 |
| 473 Laser |
Confocal |
FITC |
Cy2 |
AF488 |
487 |
519 |
| 561 Laser |
Confocal |
TRITC/TxRed |
Cy3/Cy3.5 |
AF546/594 |
561 |
578 |
| 635 Laser |
Confocal |
APC |
Cy5/Cy5.5 |
AF647 |
637 |
668 |
Objectives
NOTE: Si immersion objectives will be MOVED to the new FV4000 in Spring 2025. We will replace these with standard oil-immersion objectives
| Objective |
OIL |
Corr |
WD mm |
Z DOF µm |
| 4x/0.16 |
|
UPlan Superapochromat |
13.00 |
31.64 |
| 10x/0.4 |
|
UPlan Superapochromat |
3.10 |
5.06 |
| 30x/1.05 |
Si |
UPlan Superapochromat |
0.80 |
0.99 |
| 60x/1.30 |
Si |
UPlan Superapochromat |
0.30 |
0.57 |
| 100x/1.35 |
Si |
UPlan Superapochromat |
0.20 |
0.49 |
