Leica SP8 Spectral Confocal Microscope
Overview
- Spectral scanning confocal microscope
- White-light tunable laser and tunable detectors for flexible fluorescent imaging of up to 7 tags.
- Resonant scanning mode for fast acquisition
- Best for large stitched images
Specifications
- Leica DMi8 microscope
- Environmental Enclosure with heat and humidity.
- Leica Z-galvo and motorized XY stage
- White light laser system supplemented with brighter 405 Diode and 488 Argon laser lines
- 3 High Sensitivity Detectors and 1 PMT
- Optional transmitted light detector
- Both Galvonometer and Fast Resonant scanning options
- Images sizes up to 8192 x 8192 pixels
- Leica acquisition software
Fluorescence Filters and Lasers
White-light laser and tuneable filters for flexible fluorophore choices. Contact Jeff Wyckoff for more information.
| Name |
Laser |
Dyes |
Ex |
Em |
| 405 Laser |
Diode |
DAPI/Hoechst/BV dyes |
405 |
Tuneable |
| 458 Laser |
Argon |
various |
458 |
Tuneable |
| 476 Laser |
Argon |
various |
476 |
Tuneable |
| 488 Laser |
Argon |
various |
488 |
Tuneable |
| 496 Laser |
Argon |
various |
496 |
Tuneable |
| 514 Laser |
Argon |
various |
514 |
Tuneable |
| White Light Laser |
WLL |
various |
470-670 |
Tuneable |
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470 |
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670 |
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Objectives
| Objective |
OIL |
Corr |
WD mm |
Z DOF µm |
| 5x/0.15 |
|
HC PL FLUOTAR Semiapochromat |
13.70 |
33.11 |
| 10x/0.40 |
|
HC PL APO CS2 Apochromat |
2.56 |
5.06 |
| 25x/0.95 |
Water |
Fluotar VISIR Semiapochromat |
2.40 |
1.17 |
| 63x/1.40 |
Oil |
HC PL APO CS2 Apochromat |
0.14 |
0.54 |
| 100x/1.40 |
Oil |
HC PL APO CS2 Apochromat |
0.13 |
0.50 |
