Coming in April 2025
Overview
- Combined multiphoton/confocal microscope
- Multiphoton mode for intravital imaging
- Standard confocal mode with five lasers for imaging out to the near-IR (Cy7)
- Resonant scanning mode for fast acquisition of large stitched 3D images
- Sensitive wavelength-tunable photon-counting detectors
- Silicone immersion objectives allow deep image in thick samples [link]
Specifications
- Inverted Evident (formerly Olympus) FluoView FV4000 microscope
- MaiTai DeepSea Ti:Sapphire multiphoton laser for multiphoton acquisition
- Five standard lasers for standard confocal imaging out to near-IR (Cy7)
- Resonant confocal scanning mode for fast imaging
- XY Motorized stage with peizo Z for extremely fast 3D scanning
- 4 descanning detectors for standard confocal imaging
- 4 non-descanning detectors for multiphoton imaging
- All detectors are low-noise photon-counting detectors
- Counting individual photons gives accurate repeatable quantification of fluorescent signals
- Transmitted light detector with optional DIC optics for better contrast
- Silicone Immersion Objectives for deep tissue imaging (>500 µm)
- Heated environmental chamber with CO2 for live animal or cell imaging
- Evident acquisition software
(More specifications to come)
Fluorescence Filters and Lasers
| Name | Set | Std dyes | Cy series | Alexa | Ex | Em |
|---|---|---|---|---|---|---|
| MiTai Laser | Multiphoton | various | 690-1040 | Tuneable | ||
| 405 Laser | Std Confocal | DAPI/Hoechst | 405 | Tuneable | ||
| 488 Laser | Std Confocal | FITC | Cy2 | AF488 | 488 | Tuneable |
| 561 Laser | Std Confocal | TRITC/TxRed | Cy3/Cy3.5 | AF546/594 | 561 | Tuneable |
| 640 Laser | Std Confocal | APC | Cy5/Cy5.5 | AF647 | 640 | Tuneable |
| 730 Laser | Std Confocal | Cy7 | Cy7 | AF750 | 730 | Tuneable |
Objectives
Si immersion objectives allow deeper penetration of fluorescence
| Objective | Imm | Type | WD mm | Z DOF µm |
|---|---|---|---|---|
| 4x/0.16 | air | UPlan Extended Apochromat | 13 | 31.6 |
| 10x/0.40 | air | UPlan Extended Apochromat | 3.1 | 5.1 |
| 20x/0.80 | air | UPlan Extended Apochromat | 0.6 | 1.3 |
| 30x/1.05 | Si | UPlan Superapochromat IR | 0.8 | 1 |
| 60x/1.30 | Si | UPlan Superapochromat | 0.3 | 0.6 |
| 100x/1.35 | Si | UPlan Superapochromat | 0.2 | 0.5 |