Evident FV4000 Multiphoton and Confocal Microscope

NEW

Overview

• Combined multiphoton/confocal microscope
• Multiphoton mode for intravital imaging
• Standard confocal mode with five lasers for imaging out to the near-IR (Cy7)
• Resonant scanning mode for fast acquisition of large stitched 3D images
• Sensitive wavelength-tunable photon-counting detectors
• Silicone immersion objectives allow deep image in thick samples [link]

Specifications

• Inverted Evident (formerly Olympus) FluoView FV4000 microscope
• MaiTai DeepSea Ti:Sapphire multiphoton laser for multiphoton acquisition
• Five standard lasers for standard confocal imaging out to near-IR (Cy7)
• Resonant confocal scanning mode for fast imaging
• XY Motorized stage with peizo Z for extremely fast 3D scanning
• 4 descanning detectors for standard confocal imaging
• 4 non-descanning detectors for multiphoton imaging
• All detectors are low-noise photon-counting detectors
• Counting individual photons gives accurate repeatable quantification of fluorescent signals
• Transmitted light detector with optional DIC optics for better contrast
• Silicone Immersion Objectives for deep tissue imaging (>500 µm)
• Heated environmental chamber with CO2 for live animal or cell imaging
• Evident acquisition software

Fluorescence Filters and Lasers

Objectives

Si immersion objectives allow deeper penetration of fluorescence