Overview
- Fast Spinning-Disk confocal for live-cell imaging
- BL1 samples ONLY (fixed samples or live non-human material or cell lines)
- BL2 imaging coming fall 2026 (live human-derived cells and tissues)
LOCATION
- Located in the Building 68 Bio-Core Satellite Facility
- Contact KIMCF-68 staff Linda Melodia for assistance
Specifications
- Nikon Ti2-E Inverted motorized microscope
- Nikon XYZ Motorized stage with perfect focus module for Z stability
- Piezo-Z stage insert for high-speed 3D nanopositioning
- Stage-top environment chamber with temperature control
- 4-line laser system
- Nikon NIS Elements Acquisition software
- Yokogawa CSU-W1 spinning disk confocal head
- Hamamatsu ORCA-Quest qCMOS camera
- iXon EMCCD camera available
Fluorescent Filters and Lasers
| Name | Set | Std dyes | Cy series | Alexa | Ex | Em |
|---|---|---|---|---|---|---|
| 405 Laser | Confocal | DAPI/Hoechst/BV421 | 405 | 455/50 | ||
| 488 Laser | Confocal | FITC | Cy2 | AF488 | 488 | 525/36 |
| 561 Laser | Confocal | TRITC/TxRed | Cy3/Cy3.5 | AF546/594 | 561 | 605/52 |
| 640 Laser | Confocal | APC | Cy5/Cy5.5 | AF647 | 640 | 705/72 |
Objectives
| Objective | OIL | Corr | WD mm | Z DOF um |
|---|---|---|---|---|
| 4x/0.2 | Plan Fluor | 20.0 | 96.6 | |
| 20x/0.5 | Plan Fluor | 2.10 | 364 | |
| 60x/1.42 | Oil | Plan Apochromat Lambda D | 0.15 | 0.296 |
| 100x/1.45 | Oil | Plan Apochromat Lambda D | 0.13 | 0.754 |
Data Transfer
- USB flash storage or drives are NOT PERMITTED on core instruments.
- All data must be transferred over the network
- Use the nearby workstation in office 68-371 to transfer your data from the microscope to your server