Methods

Preclinical testing: Designing an in-vivo study

‚ÄčAnimal imaging: Visualizing structures and biological processes in vivo

  • Optical imaging
    • Fluorescence vs. bioluminescence
    • How to choose a dye
    • Minimize autofluorescent/autoluminescent background
    • Optimal settings (exposure time, pixel size, fstop, filters)
    • Advanced settings (spectral unmixing, tomography)
  • Magnetic resonance imaging
    • How does it work?
    • Pros & Cons
    • MRI, fMRI, MRS, DWI/DTI, x-nuclei
    • How to choose a pulse sequence
    • Image weighting and contrast
    • Contrast agents (Gd, iron oxide) vs. labels (fluorine)
    • Common pulse sequences
    • Useful links