Instrumentation

In-vivo instrumentation (imaging)

IVIS Spectrum bioluminescent and fluorescent imaging systems (Perkin Elmer)

IVIS spectrum instrument

Our two IVIS Spectrum instruments can perform both fluorescence and bioluminescence imaging. Typical applications include qualitative tumor burden tracking, high throughput in vivo and ex vivo biodistribution and screening studies. Systems specifications and an acquisition software manual are available. Typical features include:

Imaging throughput Up to 5 mice or 2 rats per imaging session
Heating Yes, heated platform
Physiological monitoring No
Acquisition type 2D (3D through surface topography)
Typical acquisition times 1 sec – 1 min
Nominal spatial resolution ex vivo: 20 um; in vivo: mm depending on depth in tissue
Max field of view 23 cm x 23 cm
Analysis software Living Image

Skyscan 1276 animal and specimen x-ray microtomograph (Bruker)

Skyscan 1276 instrument

Our microCT instrument can perform high resolution x-ray tomography and is typically used for bone structure imaging, lung tumor imaging, implant tracking as well as contrast enhanced vascular imaging. Typical features include:

Imaging throughput Up to 4 mice or 1 rat, multiple specimens per imaging session
Heating Yes, heated airflow
Physiological monitoring Yes (ECG, breathing, temperature)
Acquisition type 2D, 3D, 4D, helical
Typical acquisition times 1 – 40 mins
Nominal spatial resolution ex vivo: 5 um, in vivo: 20 um
Max field of view Continuously adjustable (specimen-mouse-primate)
Analysis software CTan, CTVol

Vevo 3100 and LAZR-X ultrasound and photoacoustic imaging system (FujiFilm-VisualSonics)

Vevo 3100 instrument

Our ultrasound system is equipped with four transducers with frequencies from 18 to 70 MHz ideal for imaging of a wide variety of species from zebrafish to primates. Typical applications include blood flow and capillary perfusion measurements, cardiac imaging, tumor imaging, needle guidance and implant imaging.

Our photoacoustic system is equipped with a multi-wavelength pulsed laser source (680-970 nm and 1200-2000nm) that allows for spectral characterization of compounds such as gold and carbon nanostructures, as well as oxygen saturation measurements in blood.

Typical features include:

Imaging throughput 1 mouse or rat per imaging session
Heating Yes, heated platform
Physiological monitoring Yes (ECG, breathing, temperature)
Acquisition type 2D, 3D, 4D
Typical acquisition times Real time – 5 mins for 3D
Nominal spatial resolution 30 um@70 MHz to 100 um@18 MHz
Max field of view 9.7 mm x 12 mm @ 70 MHz to 23 mm x 36 mm @18 MHz
Analysis software VevoLab

Varian (magnet) / Bruker (console) 7T MRI system (A2B Bruker upgrade)

MRI system
Imaging throughput 1 mouse or rat per imaging session
Heating Yes, heated airflow
Physiological monitoring Yes (ECG, breathing, temperature)
Acquisition type 2D, 3D
Typical acquisition times 2 – 30 mins
Nominal spatial resolution 150 um
Max field of view 40 mm
Analysis software ParaVision

G8 PET/CT preclinical imaging system (Perkin Elmer)

PET/CT instrument

Our PET system is capable of highly sensitive tumor detection and biodistribution and therapeutic targeting studies using radioisotopes such as 18F, 64Cu, 89Zr.  It is limited to full body mouse and rat head due to the small bore which allows for superb sensitivity comparatively to other PET machines. The system also includes a CT scanner that allows for co-registration of the PET image with the anatomy of the animal. System specifications are available. Typical features include:

Imaging throughput 1 mouse or rat per imaging session
Heating Yes
Physiological monitoring No
Acquisition type 2D, 3D
Typical acquisition times 10 mins (PET), 5 mins (CT)
Nominal spatial resolution 1.4 mm at the center of field of view
Max field of view 4.7 cm x 9.5 cm
Analysis software VivoQuant

Ex-vivo instrumentation (non-imaging)

2480 Wizard2 automatic gamma counter (Perkin Elmer)

2480 Wizard2 automatic gamma counter instrument

Our gamma counter is well suited for quantitative measurements of PET tracer and a large library of gamma emitting radionuclides in excised tissues and comparison with image-based PET biodistribution data. Systems specifications are available. Typical features include:

Imaging throughput 1000 samples per run
Physiological monitoring Library of 51 radionuclides
Acquisition type Counts
Sample volume 3 – 20 ml
Energy window 15-2000 keV
Analysis software StatLIA

Data Analysis Resources

The Preclinical Imaging & Testing Facility currently has 5 workstations available to users with commercially available and custom build image visualization and analysis software. 

For reserving time in any of the workstations please use iLabs. To get log in credentials please contact the facility’s staff. For data management in these workstations, users will be provided with specific instructions as part of their training.

Processor Memory Graphics Storage Software
Sonic - Workstation 1 (PC Windows)
3.2 GHz AMD
Ryzen Quad-Core
32 GB 2400
MHz GDDR5
AMD Radeon (TM) RX 570 4096 MB 1 TB Amira, Living Image, VevoLab
Positron - Workstation 2 (iMac)
3.4 GHz Intel
Core i5
32 GB 2400
MHz DDR4
Radeon Pro 570 4096 MB 1 TB VivoQuant 3, Amide, ImageJ
Photon - Workstation 4 (PC Windows)
Dual Intel Xeon Gold 6136 12 core, 3.0 GHz 192 GB Nvidia Quadro RTX 5000, 6GB 10 TB Matlab, Julia, MicroView, ImageJ, NRecon


Software User Guides