Specifications
- Inverted Olympus X71 microscope with XYZ nano-motion stage with 18 mm of XY travel
- Environmental chamber with temperature control and CO2 enrichment
- Mercury lamp illumination coupled to a fiber optic cable
- Halogen transmitted light source
- Objectives: 10X, 20X, 40X, 60X, 100X
- Softworx software for image acquisition and deconvolution
- Photometrics CoolSNAP HQ camera
Fluorescence filter sets
| Excitation-Emission | Probes & Fluorochromes | Sample |
|---|---|---|
| 360/457 | DAPI, Hoechst, Coumarin | Fixed cells |
| 488/528 | FITC, Alexa488, GFP | Fixed/live cells |
| 555/617 | Rhodamine, Alexa555, Texas Red, Phycoerythrin | Fixed cells |
| 647/685 | Cy5, ALEXA 647 | |
| 436/465 | CFP | Live cells |
| 470/525 | EGFP | Live cells |
| 492/535 | YFP | Live cells |
| 580/630 | DsRed Express, DsRed2 | Live cells |